Hyper-reactive mononuclear cells and neutrophils in chronic periodontitis
Objectives: Stimulated mono- and polymorphonuclear cells from patients with periodontitis have shown increased release of interleukin-1b (IL-1b) and oxygen radicals, respectively. The aim was to study whether this hyper-reactivity could be found both in mono- and polymorphonuclear cells from the...
| Main Author: | A. Gustafsson |
|---|---|
| Format: | Koleksi Audio Visual |
| Language: | Bahasa Inggris |
| Published: |
Blackwell Synergy
2006
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| Subjects: | |
| Online Access: |
http://oaipmh-jogjalib.umy.ac.idkatalog.php?opo=lihatDetilKatalog&id=94064 |
| Summary: |
Objectives: Stimulated mono- and polymorphonuclear cells from patients with
periodontitis have shown increased release of interleukin-1b (IL-1b) and oxygen
radicals, respectively. The aim was to study whether this hyper-reactivity could be
found both in mono- and polymorphonuclear cells from the same patient, and whether
there was a relation to the gene coding for IL-1b (IL-1b13953).
Material and Methods: Peripheral mononuclear cells from 14 non-smoking and
well-treated patients and pair-matched controls were incubated with opsonized
Staphylococcus aureus and lipopolysaccharide (LPS). Released IL-1b and tumour
necrosis factor (TNF)-a were determined with ELISA. Generation of oxygen radicals
from the Fcg-receptor-stimulated neutrophils was measured with chemiluminescence
and the polymorphism at IL-1b13953 was measured with polymerase chainreaction.
Results: The mononuclear cells from the patients released more IL-1b after
incubation with LPS (po0.001) and with bacteria (po0.05). The release of TNF-a
tended to be higher in the patient group. The peripheral neutrophils from the patients
generated more oxygen radicals (po0.06). We found no differences between the study
groups regarding the IL-1b13953 polymorphism.
Conclusion: The similarity in systemic inflammation between patients and controls
suggests that the increased release/generation of IL-1b and oxygen radicals from
peripheral leukocytes in periodontitis patients is of a constitutional nature and of
pathogenic relevance. |
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