Transforming growth factor- b stimulates Interleukin-11 production by human periodontal ligament and gingival fibroblasts
Background: Transforming growth factor (TGF)-b is a potent multifunctional polypeptide, abundant in the bone matrix. Interleukin (IL)-11 is a pleiotropic cytokine with effects on multiple cell types. The present study was performed to evaluate the regulatory effects of TGF-b on IL-11 production b...
| Main Author: | R. Yashiro |
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| Format: | Koleksi Audio Visual |
| Language: | Bahasa Inggris |
| Published: |
journal of clinical periodontology
2006
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| Subjects: | |
| Online Access: |
http://oaipmh-jogjalib.umy.ac.idkatalog.php?opo=lihatDetilKatalog&id=94070 |
| Summary: |
Background: Transforming growth factor (TGF)-b is a potent multifunctional
polypeptide, abundant in the bone matrix. Interleukin (IL)-11 is a pleiotropic cytokine
with effects on multiple cell types. The present study was performed to evaluate the
regulatory effects of TGF-b on IL-11 production by human periodontal ligament cells
(PDL) and human gingival fibroblasts (HGF).
Material and Methods: The expression of TGF-b receptor in PDL and HGF were
observed using flow cytometry. PDL and HGF were stimulated with TGF-b with or
without protein kinase C (PKC) inhibitors and activator. IL-11, bone morphogenetic
protein-2 (BMP-2) and TGF-b mRNA expression was quantified by real-time
polymerase chain reaction (PCR). IL-11 production was measured using enzymelinked
immunosorbent assay.
Results: PDL and HGF expressed both TGF-b receptor I and TGF-b receptor II on the
cell surfaces. IL-11 mRNA expression and IL-11 production were augmented by TGFb
in both PDL and HGF, with higher values in PDL. PKC inhibitors partially suppressed
TGF-b-induced IL-11 production in PDL and HGF, whereas activator enhanced it.
TGF-b mRNA and BMP-2 mRNA expression were up-regulated by TGF-b in PDL.
Conclusion: These results suggest that PDL produce IL-11 in response to TGF-b. |
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